Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ MeltDoctor™ HRM Positive Control Kit
Provides nucleic acid controls to demonstrate and troubleshoot High Resolution Melt (HRM) analysis
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ PureQuant CD3+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystems™ TaqMan™ Advanced miRNA Human A and B 96-well Plates, standard
The Applied Biosystems TaqMan Advanced miRNA Human A and B 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format.
Applied Biosystems™ PureQuant CD4+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Thermo Scientific™ SureTect™ Campylobacter jejuni, C. coli and C. lari PCR Assay
Rapidly detect Campylobacter jejuni, C. coli and C. lari in food and environmental samples.
Applied Biosystems™ TaqMan™ QSY and QSY2 Probes
Applied Biosystems TaqMan QSY and QSY2 probes are dual-labeled probes used for real-time PCR. TaqMan QSY and QSY2 Probes are designed for optimal sensitivity and performance in multiplexed qPCR experiments for detection of up to 6 targets.
Applied Biosystems™ resDNASEQ™ dPCR HEK293 DNA Kit
The Applied Biosystems resDNASEQ dPCR HEK293 DNA Kit is a digital PCR (dPCR)-based solution to quantitate residual DNA from HEK293 expression systems, which are commonly used for production of gene therapies, cell-based vaccines, and similar therapeutics.
Applied Biosystems™ TrueMark™ PGx Comprehensive Plasmid Controls
TrueMark PGx Comprehensive Plasmid Controls contains three linearized multi-target plasmid pools with human genome target sequences commonly used for pharmacogenomic research testing.
Applied Biosystems™ ViralSEQ™ dPCR AAV Titer Kit
The Applied Biosystems ViralSEQ dPCR AAV Titer Kit is a digital PCR (dPCR) assay for the reproducible quantitation of AAV particles independent of the serotype.
Thermo Scientific™ SureTect™ Salmonella Infantis PCR Assay
Rapidly detect Salmonella Infantis in carcass rinses and poultry part samples with the Thermo Scientific™ SureTect™ Salmonella Infantis PCR Assay workflow.
Applied Biosystems™ VetMAX™-Plus Multiplex One Step RT-PCR Kit
For use with VetMAX™ Reagents and VetMAX™ Controls.
Applied Biosystems™ Path-ID™ Multiplex One-Step Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ Path-ID™ Multiplex One-Step RT-PCR Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
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Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).